Publications associées à Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Didier Trono, Priscilla Turelli, Florence Pojer, Charlène Mireille Raymonde Raclot, Valeria Cagno, Jérémy Campos, Céline Pellaton

The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies in the serum of an individual indicates previous infection or vaccination. However, it provides limited insight into the protective nature of this immune resp ...

AMER ASSOC ADVANCEMENT SCIENCE2021

Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays

Nathalie Brandenberg, Camilla Carla Alma Ceroni

Background The pathways that control protein transport across the blood-brain barrier (BBB) remain poorly characterized. Despite great advances in recapitulating the human BBB in vitro, current models are not suitable for systematic analysis of the molecul ...

BMC2021

DuoMab: a novel CrossMab-based IgG-derived antibody format for enhanced antibody-dependent cell-mediated cytotoxicity

Henning Paul-Julius Stahlberg

High specificity accompanied with the ability to recruit immune cells has made recombinant therapeutic antibodies an integral part of drug development. Here we present a generic approach to generate two novel IgG-derived antibody formats that are based on ...

Informa UK Limited2019

Targeting small molecule drugs to T cells with antibody-directed cell-penetrating gold nanoparticles

Francesco Stellacci, Ye Yang, Ahmet Bekdemir

We sought to develop a nanoparticle vehicle that could efficiently deliver small molecule drugs to target lymphocyte populations. The synthesized amphiphilic organic ligand-protected gold nanoparticles (amph-NPs) were capable of sequestering large payloads ...

2019

Tumor-targeted 4-1BB agonists for combination with T cell bispecific antibodies as off-the-shelf therapy

Henning Paul-Julius Stahlberg, Thomas Hofer, Wei Xu

Endogenous costimulatory molecules on T cells such as 4-1BB (CD137) can be leveraged for cancer immunotherapy. Systemic administration of agonistic anti-4-1BB antibodies, although effective preclinically, has not advanced to phase 3 trials because they hav ...

American Association for the Advancement of Science (AAAS)2019

Two-Dimensional Label-Free Affinity Analysis of Tumor-Specific CD8 T Cells with a Biomimetic Plasmonic Sensor

Hatice Altug, George Coukos, Xiaokang Li, Aurélian Michel John-Herpin, Maria Soler Aznar

The screening and analysis of T cells functional avidity for specific tumor-associated antigens is crucial for the development of personalized immunotherapies against cancer. The affinity and kinetics of a T cell receptor (TCR) binding to the peptide-major ...

AMER CHEMICAL SOC2018

An electrochemical sensor for quantitative analysis of Rhesus D antibodies in blood

Giovanni De Micheli, Sandro Carrara, Irene Taurino, Francesca Criscuolo, Tugba Kilic

The Hemolytic Disease of the Fetus and Newborn (HDFN), if untreated, can have severe consequences on baby’s health, eventually causing death. Prevention and diagnosis of pregnancies at risk of HDFN consists in quantitative analysis in hospital every 2-4 we ...

IEEE2017

Simulation and Theory of Antibody Binding to Crowded Antigen-Covered Surfaces

Paolo De Los Rios, Giuseppe Foffi, Francesco Piazza

In this paper we introduce a fully flexible coarse-grained model of immunoglobulin G (IgG) antibodies parametrized directly on cryo-EM data and simulate the binding dynamics of many IgGs to antigens adsorbed on a surface at increasing densities. Moreover, ...

Public Library Science2016

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak

Transient gene expression (TGE) in human embryonic kidney (HEK-293) and Chinese hamster ovary (CHO) cells is a well-established technology for the rapid generation of recombinant proteins. Although the maximum TGE yields have reached 1 g/L or more, the amo ...

American Chemical Society2015

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Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays

DuoMab: a novel CrossMab-based IgG-derived antibody format for enhanced antibody-dependent cell-mediated cytotoxicity

Targeting small molecule drugs to T cells with antibody-directed cell-penetrating gold nanoparticles

Tumor-targeted 4-1BB agonists for combination with T cell bispecific antibodies as off-the-shelf therapy

Introducing tolerance to foreign and partly foreign proteins by erythrocyte binding and artificial glycosylations

Two-Dimensional Label-Free Affinity Analysis of Tumor-Specific CD8 T Cells with a Biomimetic Plasmonic Sensor

An electrochemical sensor for quantitative analysis of Rhesus D antibodies in blood

Simulation and Theory of Antibody Binding to Crowded Antigen-Covered Surfaces

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

Graph Chatbot

Chattez avec Graph Search

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

Targeting small molecule drugs to T cells with antibody-directed cell-penetrating gold nanoparticles

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Simulation and Theory of Antibody Binding to Crowded Antigen-Covered Surfaces

Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays

DuoMab: a novel CrossMab-based IgG-derived antibody format for enhanced antibody-dependent cell-mediated cytotoxicity

Tumor-targeted 4-1BB agonists for combination with T cell bispecific antibodies as off-the-shelf therapy

Introducing tolerance to foreign and partly foreign proteins by erythrocyte binding and artificial glycosylations

An electrochemical sensor for quantitative analysis of Rhesus D antibodies in blood

Two-Dimensional Label-Free Affinity Analysis of Tumor-Specific CD8 T Cells with a Biomimetic Plasmonic Sensor