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A bio-electronics system that measures cytosolic [Ca2+] in vitro in the INS-1E pancreatic beta-cells using a 430 nm excitation light with the excitation wavelength of the probe being at 435 nm was successfully implemented and tested. We transfected the INS-1E cells with a plasmid that is responsible for the FRET based [Ca2+] protein sensor complex, that emit light at both 485 nm and 535 nm. The F10 cell based biosensor and its working principle are described with protein structure homology modeling. The efficacy of absorption angle independent filters atop the photo-detector is demonstrated with a 1163% improvement in SNR. Oxygenation of cells alongside stimulant circulation is possible with the aid of a magnetic stirrer. The system successfully monitors glucose continuously with 2.5 mM stimulation. Continuously glucose monitoring with periodic 2.5 mM glucose stimulation and binary 11 mM glucose vs. KRBH were successful showing clear repeatable ratio changes.
Ardemis Anoush Boghossian, Hanxuan Wang, Vitalijs Zubkovs
Sandro Carrara, Nattakarn Wuthibenjaphonchai