Publication
Automated High-Content Phenotyping Of The Nematode C. Elegans At Single Animal Resolution With A Microfluidic Platform
Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang
2019
Article de conférence
2019
Article de conférence
Résumé
In this work, we present a novel microfluidic approach to culture the nematode C. elegans from the onset of embryogenesis to the adult stage at single animal resolution. Our device allows high-throughput experimentation, high-resolution imaging, thereby providing high-content phenotyping in a highly parallel fashion. As a validation study, we studied the dose-dependent influence of the anthelmintic drug, tetramisole on the nematodes' development during their full life cycle. Our results revealed a clear impact of tetramisole on various worm phenotypes.
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Proximité ontologique
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High-content screening (HCS), also known as high-content analysis (HCA) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell in a desired manner. Hence high content screening is a type of phenotypic screen conducted in cells involving the analysis of whole cells or components of cells with simultaneous readout of several parameters.
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