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An optical pancreatic beta-cell based biosensor for measuring cytosolic [Ca2+] is presented in this work. An in vitro bio-electronic system is built for testing various applications and characterizing features of the biosensor. A panoply of applications such as effect of temperature and response of the cells in culture media with 11 mM glucose vs. KRBH with 0 mM glucose are explored. The effect of the excitation light intensity and integration time on the fluorescence performance of the biosensor FRET probe are elucidated experimentally.
Sandro Carrara, Nattakarn Wuthibenjaphonchai
Ardemis Anoush Boghossian, Hanxuan Wang, Vitalijs Zubkovs
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