Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy
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Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...
The evolution path of nanotechnologies moves through the domain of microscopy. Among all the kinds of imaging tools available today, optical imaging systems are of primary importance. When using optical imaging systems, we take profit of the information re ...
Institut de Microtechnique, Université de Neuchâtel2006
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...
Several recent studies have shown that fluorescent particles can be localized with an accuracy that is well beyond traditional resolution limits. Using a theoretical model of the image formation process that accounts for possible sources of noise, Cramer-R ...
Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2007
Several recent studies have shown that fluorescent particles can be localized with an accuracy that is well beyond traditional resolution limits. Using a theoretical model of the image formation process that accounts for possible sources of noise, Cramér-R ...
Recent developments in Digital Holographic Microscopy (DHM) have permitted to achieve imaging accuracies and resolutions down to the nano-range. This result could be obtained by careful control of all parameters involved in the wavefront reconstruction. ...
Spatial, energy, and time-dependent effects induced by surface charging of conductive and nonconductive samples have been studied by spectroscopic and microscopic techniques. Surface charging of indium-tin oxide and cesium iodide has been studied by atomic ...
In this thesis a new approach for single molecule detection and analysis is explored. This approach is based on the combination of two well established methods, fluorescence correlation spectroscopy (FCS) and total internal reflection fluorescence microsco ...
We present an iterative deconvolution algorithm that minimizes a functional with a non-quadratic wavelet-domain regularization term. Our approach is to introduce subband-dependent parameters into the bound optimization framework of Daubechies et al.; it is ...
