Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...
The idea of iterative process optimization based on collected output measurements, or "real-time optimization" (RTO), has gained much prominence in recent decades, with many RTO algorithms being proposed, researched, and developed. While the essential goal ...
Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...
Adaptive Optics (AO) improves the efficiency of the optical devices in confocal imaging systems by reducing wavefront aberrations. Aberration is caused by imperfections within the system and reduces the optical signal to noise ratio of the resultant images ...
To meet strict requirements of the information society technologies, antennas and circuit elements are becoming increasingly complex. Frequently, their electromagnetic (EM) properties cannot be anymore expressed in closed-form analytical expressions mainly ...
We present a multilevel extension of the popular "thresholded Landweber" algorithm for wavelet-regularized image restoration that yields an order of magnitude speed improvement over the standard fixed-scale implementation. The method is generic and targete ...
The subject of this thesis is image restoration, that is, deconvolution and denoising. Our work is motivated by applications in the rapidly expanding field of biological imaging and in particular fluorescence microscopy. Inverse problems in this area typic ...
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For good performance in practice, real-time optimization schemes need to be able to deal with the inevitable model-mismatch problem. Unlike the two-step schemes combining parameter estimation and optimization, the modifier-adaptation approach uses experime ...
Tracking algorithms are traditionally based on either a variational approach or a Bayesian one. In the variational case, a cost function is established between two consecutive frames and minimized by standard optimization algorithms. In the Bayesian case, ...
Tracking algorithms are traditionally based on either a variational approach or a Bayesian one. In the variational case, a cost function is established between two consecutive frames and minimized by standard optimization algorithms. In the Bayesian case, ...
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