Spatio-temporal modeling of the crowding conditions and metabolic variability in microbial communities
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Background: Metabolic reconstructions (MRs) are common denominators in systems biology and represent biochemical, genetic, and genomic (BiGG) knowledge-bases for target organisms by capturing currently available information in a consistent, structured mann ...
Using the transient interleukin (IL)-2 secretion of effector T helper (Teff) cells as an example, we show that self-organizing multicellular behavior can be modeled and predicted by an adaptive gene network model. Incorporating an adaptation algorithm we e ...
Hydrophilic nanocarriers, formed by electrostatic interaction between their oppositely charged components, are recognized as nanomaterials with an enormous potential as vectors in biomedical and pharmaceutical applications. However, comprehensive informati ...
The objective of this thesis was to characterize the growth stoichiometry, the specific recombinant protein productivity and the regulation of the alcohol oxidase (AOX) enzyme of a Pichia pastoris Mut+ strain, performing conventional chemostat cultures and ...
Objectives To develop a high throughput screening-compatible assay for the selection of species-specific antibiotics that do not harm human cells. Methods Staphylococcus aureus and human reporter cells continuously generating a fluorescence signal were com ...
We introduce bounded asynchrony, a notion of concurrency tailored to the modeling of biological cell-cell interactions. Bounded asynchrony is the result of a scheduler that bounds the number of steps that one process gets ahead of other processes; this all ...
During infection, most pathogenic bacteria deliver proteins to the host cell cytoplasm to manipulate host behavior. In this issue of Cell Host & Microbe, Spanò and colleagues describe a system where a bacterium produces an exotoxin while inside the host ce ...
Synthetic bioactive hydrogels have been widely recognized as key elements of emerging strategies to engineer tissues. However, the current shortage of highly specific and biocompatible methods to form and functionalize these materials hampers their wide ph ...
Notch1 signaling is required for T cell development and has been implicated in fate decisions in the thymus. We showed that Notch1 deletion in progenitor T cells (pro-T cells) revealed their latent developmental potential toward becoming conventional and p ...
The aim of this contribution is to review the application of thermodynamics to live cultures of microbial and other cells and to explore to what extent this may be put to practical use. A major focus is on energy dissipation effects in industrially relevan ...