Publication
Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel
Publications associées (61)
Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1
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In most metazoans, centrioles are lost during oogenesis, ensuring that the zygote is endowed with the correct number of two centrioles, which are paternally contributed. How centriole architecture is dismantled during oogenesis is not understood. Here, we ...
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Microtubules, a critical component of the cytoskeleton, carry post-translational modifications (PTMs) that are important for the regulation of key cellular processes. Long-lived microtubules, in neurons particularly, exhibit both detyrosination of a-tubuli ...
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The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...
EPFL2022TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin
Pierre Gönczy, Coralie Busso, Benita Wolf, Fernando Romero Balestra, Tessa Vivianne Averink, Alizée Buff
TRIM37 is an E3 ubiquitin ligase mutated in Mulibrey nanism, a disease with impaired organ growth and increased tumor formation. TRIM37 depletion from tissue culture cells results in supernumerary foci bearing the centriolar protein Centrin. Here, we chara ...
ELIFE SCIENCES PUBLICATIONS LTD2021A helical inner scaffold provides a structural basis for centriole cohesion
Henning Paul-Julius Stahlberg, Davide Gambarotto, Paul Guichard, Virginie Hamel
The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary ...
2020Centriole foci persist in starfish oocytes despite Polo-like kinase 1 inactivation or loss of microtubule nucleation activity
Pierre Gönczy, Nils Kalbfuss, Marie Juliette Ségolène Pierron
Centrioles must be eliminated or inactivated from the oocyte to ensure that only the two functional centrioles contributed by the sperm are present in the zygote. Such removal can occur during oogenesis, as in Drosophila, where departure of Polo kinase fro ...
2020Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish
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Oriented cell division is a fundamental mechanism to control asymmetric stem cell division, neural tube elongation and body axis extension, among other processes. During zebrafish gastrulation, when the body axis extends, dorsal epiblast cells display divi ...
NATURE PUBLISHING GROUP2020Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography
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Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...
2020MondoA regulates gene expression in cholesterol biosynthesis-associated pathways required for zebrafish epiboly
Cédric Gobet, Frédéric Bruno Martin Gachon, Benjamin Dieter Weger, Meltem Weger
The glucose-sensing Mondo pathway regulates expression of metabolic genes in mammals. Here, we characterized its function in the zebrafish and revealed an unexpected role of this pathway in vertebrate embryonic development. We showed that knockdown of mond ...
2020Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails
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Microtubule doublets (MTDs), consisting of an incomplete B-microtubule at the surface of a complete A-microtubule, provide a structural scaffold mediating intraflagellar transport and ciliary beating. Despite the fundamental role of MTDs, the molecular mec ...
AMER ASSOC ADVANCEMENT SCIENCE2019