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Stimulus-responsive assembly of nonviral nucleocapsids

Publications associées (60)

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Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

Christoph Merten, Katarzyna Irena Gorska

Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorh ...

2009

More effective therapies are urgently needed against hepatitis C virus (HCV), a major cause of viral hepatitis. We used in vitro protein expression and microfluidic affinity analysis to study RNA binding by the HCV transmembrane protein NS4B, which plays a ...

2008

The crystal structure of the Escherichia coli RNase E apoprotein and a mechanism for RNA degradation

RNase E is an essential bacterial endoribonuclease involved in the turnover of messenger RNA and the maturation of structured RNA precursors in Escherichia coli. Here, we present the crystal structure of the E. coli RNase E catalytic domain in the apo-stat ...

Elsevier2008

The ends of linear eukaryotic chromosomes, telomeres, distinguish natural chromosome ends from DNA double stranded breaks and thus promote genome stability. Telomeres comprise a repetitive DNA skeleton, which is wrapped in specific protein complexes. Recen ...

2008

Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR

Stewart Cole, Iain Old

BACKGROUND: Phi29 polymerase based amplification methods provides amplified DNA with minimal changes in sequence and relative abundance for many biomedical applications. RNA virus detection using microarrays, however, can present a challenge because phi29 ...

2008

Characterization of APOBEC3G binding to 7SL RNA

Didier Trono, Bastien Mangeat

ABSTRACT: Human APOBEC3 proteins are editing enzymes that can interfere with the replication of exogenous retroviruses such as human immunodeficiency virus (HIV), hepadnaviruses such as hepatitis B virus (HBV), and with the retrotransposition of endogenous ...

2008

New efficient synthetic routes toward functionalised polyketides

Gérald Coste

RNA plays key roles in essential biological processes, such as protein synthesis, transcriptional regulation, splicing and retroviral replication. The structural diversity of RNA molecules and the lack of known RNA repair mechanisms make these biopolymers ...

EPFL2007

Synthesis and evaluation of 2'-O-substituted oligoribonucleotide analogues with new properties

Luc Reymond

During a previous, exploratory project in our group, it was observed that duplexes formed by oligonucleotides modified with polar 2'-O-substituents were more tolerant towards the addition of 1,2-dimethoxyethane (DME) than RNA and DNA duplexes [1]. Inspired ...

EPFL2006

A detailed characterization of the time scales and mechanisms by which RNA molecules change their folding structure upon binding of metabolites or during catalysis is important for the understanding of the different biological functions of RNA. The folding ...

EPFL2006

Synthesis of modified nucleosides for the incorporation into tRNAs

Sébastien Porcher

More than 100 modified nucleosides have been found in naturally occurring RNA sequences. These modifications are involved in the correct folding, in the maintenance of the reading frame during translation and in the proper interaction with enzymes and prot ...

EPFL2006