On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification
Graph Chatbot
Chattez avec Graph Search
Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.
AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.
Current proteomic strategies depend strongly on the development of analytical methodologies and instrumentation. In parallel to the development of mass spectrometry (MS) - based proteomic workflows, microfluidic devices emerged in this field as a flexible ...
Ionic liquids pose challenges and provide solutions for electrospray ionisation mass spectrometry (ESI-MS). Being inherently charged, they are themselves easy to characterise in both positive- and negative-ion mode by ESI-MS, but the challenge arises when ...
American Chemical Soc, Distribution Office, Dept 225, 1155 16Th St Nw, Washington, Dc 20036 Usa2009
Cysteine-containing peptide oxidation was studied both by using an inert platinum electrode and a sacrificial electrode (copper or zinc) generating metallic ions in electrospray ionization mass spectrometry (ESI-MS). Using peptides containing one, two and ...
A novel ruthenium(II) compound, trans-cis-cis-[Ru(II)Cl-2(DMSO)(2)(2-amino-5-methyl-thiazole)(2)], (I), PMRu52 hereafter, that may be obtained from the previously described (cis and trans)[Ru(II)Cl-2(DMSO)(4)] complexes, was designed, synthesized and chara ...
Metal ions are essential elements in all living systems. Their detections and identifications in biological processes require different methodologies. Mass spectrometry is one of the commonly used techniques in bioinorganic analyses to fulfill these requir ...
The present work shows that the electrochemical properties of electrospray ionization (ESI) can be used to add functions to the process. As example, we show how the choice of the electrode material can be used to study interactions between metal ions and b ...
The vacuum deposition of complex functional molecules and nanoparticles by thermal sublimation is often hindered due to their extremely low vapor pressure. This especially impedes the application of ultrahigh vacuum (UHV) based analytical and surface modif ...
Electrospray ionisation (ESI) is a soft technique used in mass spectrometry (MS) analysis able to introduce a wide variety of analytes in the gas phase. Briefly, it consists in the application of a high- voltage to a solution to spray it through a small or ...
A scanning electrochemical microscopy (SECM) protein detection methodology has been developed based on the tagging of free cysteines and other nucleophiles in proteins and peptides by benzoquinone. The tagged proteins are detected by the mediated reduction ...
In the context of prefractionation methods for proteomics, this work deals mainly with the development of electrophoretic tools for isoelectric focusing of peptides and proteins for the analysis of biological mixtures. In the light of existing devices for ...
