A unified stoichiometric model for oxidative and oxidoreductive growth of yeasts
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Heat produced by hybridoma suspension cultures was continuously monitored in a 2-L heat-flux calorimeter. Heat measurements reflected changes in the metabolic state of the culture under different aeration conditions. A highly anaerobic metab. was indicated ...
M. thermoautotrophicum was grown on a defined mineral salts medium under strictly anaerobic conditions with H2 and CO2 as the sole energy and C source, resp. The cultivation medium was optimized with respect to inorg. components, including Se(IV), W(VI), N ...
Four models taken from the literature, which permit calcn. of heats of combustion from elemental anal., are evaluated from a theor. point of view. In order to obtain exptl. values of heat of combustion with a higher degree of accuracy than that in the lite ...
A modified bench scale calorimeter has been employed to det. the heat generated by various microbial strains growing on a range of different substrates, covering degrees of redn. from 3 to 6.13. The results are analyzed and interpreted in the light of coup ...
We have used monospecific antibodies against three ribonuclease H enzymes of Saccharomyces cerevisiae to investigate their intracellular localization. Fractionation experiments, as well as immunocytochemical staining, revealed a predominantly cytoplasmic l ...
The whi2 mutation in S. cerevisiae allows mutant cells to grow with a higher specific rate and at smaller cell sizes at the onset of EtOH metab. in diauxic growth. S. cerevisiae Whi2+ and whi2 were cultivated in aerobic and anaerobic batch cultures in the ...
Fundamental aspects of chemostat cultures are reviewed. Using yeast cultures as examples, it is shown that steady states in chemostats may be predicted quant. by combining the correct no. of unstructured kinetic models with expressions for existing stoichi ...
During the anaerobic batch cultivation of Kluyveromyces fragilis on a complex medium contg. glucose (100 g/L), EtOH was produced at levels resulting in the inhibition of growth and EtOH formation. Continuous extn. of the EtOH using transmembrane distn. res ...
The growth physiol. of S. cerevisiae strains H1022 and Whi2+ was studied in aerobic batch and continuous (chemostat) cultures. Results from the measurement of biomass and medium components (off-line) together with O2, CO2, and heat measurements (online) we ...
The heat generated by both batch and continuous cultures of the yeast Kluyveromycs fragilis was studied using a modified bench scale calorimeter. Batch cultures were used to measure the heat dissipation rates and the heat yields during fully aerobic and co ...