Calorimetry and energetic efficiencies in aerobic and anaerobic microbial growth
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A review, with .apprx.43 refs. A review of different types of biol. reaction calorimetry systems currently used together with the operating principles is presented. The av. resoln. of these systems is approx. 20 to 1000 mW l-1, sufficient for studies of a ...
The invention provides methods and materials related to the prodn. of org. products. Specifically, the invention provides various recombinant yeast cells, methods for culturing yeast cells, methods for making yeast cells, nucleic acid constructs, and metho ...
Bench-scale heat flux calorimetry is applied to monitor complex microbial systems producing secondary metabolites and having a rheol. complex behavior. With such systems, biol. metabolic activity can only be measured accurately if calorimetry is improved b ...
The radical addn. co-polymn. of 2-hydroxyethyl methacrylate and Bu acrylate is carried out in a continuously operating Buss-Kneader. To calc. temp. profiles over the length of the kneader the heat balance is solved. For the heat transfer coeff. a model bas ...
Chemostat cultures of an aerobic fermenting yeast (Saccharomyces cerevisiae) and an aerobic respiring yeast (Kluyveromyces fragilis) were grown under dual C + N limitation in an isothermal reaction calorimeter. The dual limitations resulted in uncoupled ox ...
In addn. to online detection of events in biotechnol., calorimetry could be used to control processes. In the latter case, a correct and instantaneous heat signal must be available. This would be possible only if a correct evaluation of baseline evolution ...
The whi2 mutation in S. cerevisiae allows mutant cells to grow with a higher specific rate and at smaller cell sizes at the onset of EtOH metab. in diauxic growth. S. cerevisiae Whi2+ and whi2 were cultivated in aerobic and anaerobic batch cultures in the ...
Synchronized oscillating cultures of Saccharomyces cerevisiae CBS 426 exhibit periodic oxidoreductive metab. The changing metab. is detectable by in situ heat-flux calorimetry in the BSC 81, which was operated as a chemostat in these expts. The measured me ...
Yeast strains were examd. for their ability to produce glycerol, and Kluyveromyces marxianus NRRLY-665 was chosen for further optimization due to its comparatively high growth rate (0.15/h) and glycerol yield (9.5% by wt. on lactose) at 37 Deg and pH 7. Op ...
The heat generated by both batch and continuous cultures of the yeast Kluyveromycs fragilis was studied using a modified bench scale calorimeter. Batch cultures were used to measure the heat dissipation rates and the heat yields during fully aerobic and co ...
