Metabolic network analysis of a hybridoma cell line using mass balances and 14C-labeled glucose and glutamine
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The understanding of dynamic metabolic regulations is important for physiol. studies and strain characterization tasks. The present study combined transient expts. with online metabolic flux anal. (MFA) in order to quantify metabolic regulations, namely ca ...
Study of a metabolic network with respect to the sensitivity of the metabolite concentrations and the metabolic fluxes to genetic, biochemical, and environmental variations requires knowledge of the kinetic properties of the enzymes in the network. Experim ...
ABSTRACT A new form of metabolic flux analysis (MFA) called thermodynamics-based metabolic flux analysis (TMFA) is introduced with the capability of generating thermodynamically feasible flux and metabolite activity profiles on a genome scale. TMFA involve ...
A multitude of metabolic regulations occur in yeast, particularly under dynamic process conditions, such as under sudden glucose excess. However, quantification of regulations and classification of yeast strains under these conditions have yet to be elucid ...
Growth of Methanobacterium thermoautotrophicum, an anaerobic archaebacterium using methanogenesis as the catabolic pathway, is characterized by large heat prodn. rates, up to 13 W g-1, and low biomass yields, in the order of 0.02 C-mol mol-1 H2 consumed. T ...
Yeast metab. has been used extensively in scientific investigations and industrial applications. Understanding the properties of the yeast metabolic network is crucial, yet unaccomplished due to its high complexity, the different culture conditions, and th ...
Advancement of genomic research and anal. techniques enables an in-depth understanding of individual components in the cellular metab. The crit. subject is the integration of massive exptl. details and the quantification of the contributions of individual ...
The pentose phosphate pathway and the pyruvate shunt were identified as major pathways of glucose catabolism in a recombinant, riboflavin-producing Bacillus subtilis strain. Reactions connecting the tricarboxylic acid cycle and glycolysis, catalyzed by the ...
Carotenoid prodn. by microorganisms, as opposed to chem. synthesis, could fulfill an ever-increasing demand for 'all natural' products. The yeast Phaffia rhodozyma has received considerable attention because it produces the red pigment astaxanthin, commonl ...
The calorimetric response of the yeast Kluyveromyes fragilis was investigated for growth in continuous culture where N limitation was imposed on a C-limited culture. Calorimetric measurements were combined with off gas anal., measurements of biomass, subst ...