Publication
A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro
Publications associées (41)
Rescue of secretion of rare-disease-associated misfolded mutant glycoproteins in UGGT1 knock-out mammalian cells
Endoplasmic reticulum (ER) retention of misfolded glycoproteins is mediated by the ER-localized eukaryotic glycoprotein secretion checkpoint, UDP-glucose glycoprotein glucosyl-transferase (UGGT). The enzyme recognizes a misfolded glycoprotein and flags it ...
Hoboken2024Direct observation of glycans bonded to proteins and lipids at the single-molecule level
Klaus Kern, Stephan Rauschenbach
Proteins and lipids decorated with glycans are found throughout biological entities, playing roles in biological functions and dysfunctions. Current analytical strategies for these glycan-decorated biomolecules, termed glycoconjugates, rely on ensemble-ave ...
Washington2023Combining ion mobility spectrometry and cryogenic ion spectroscopy with enzymatic digestion/synthesis for the study of N-linked glycans
Glycans play an essential role in numerous physiological and pathological processes of living organisms. Despite their significant biological relevance, glycobiology remains one of the least explored fields of biochemistry. The intrinsic isomeric complexit ...
EPFL2023Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal
The structural complexity of glycans makes their characterization challenging, not only because of the presence of various isomeric forms of the precursor molecule but also because the fragments can themselves be isomeric. We have recently developed an IMS ...
AMER CHEMICAL SOC2023Identification of N-glycan positional isomers by combining IMS and vibrational fingerprinting of structurally determinant CID fragments
Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal
While glycans are present on the surface of cells in all living organisms and play key roles in most biological processes, their isomeric complexity makes their structural characterization challenging. Of particular importance are positional isomers, for w ...
ROYAL SOC CHEMISTRY2022A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies
Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Vasyl Yatsyna, Natalia Yalovenko
Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity. Recent studies have demonstrated that glycan isomers with the termi ...
ROYAL SOC CHEMISTRY2021Analyzing glycans cleaved from a biotherapeutic protein using a combination of ion mobility spectrometry and cryogenic ion spectroscopy
The characterization of biomolecules plays a central role in biomedical research. The development of state-of-the-art technologies is needed to make such analyses faster, more sensitive, and more accurate. While mass spectrometry continues to play a centra ...
EPFL2021Thioredoxin-Related Transmembrane Proteins: TMX1 and Little Brothers TMX2, TMX3, TMX4 and TMX5
The endoplasmic reticulum (ER) is site of synthesis and maturation of membrane and secretory proteins in eukaryotic cells. The ER contains more than 20 members of the Protein Disulfide Isomerase (PDI) family. These enzymes regulate formation, isomerization ...
2020Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans
Laurent Gillet, Christian Sieben
Virus infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and cell-surface receptors. Various cell-surface glycans func ...
ANNUAL REVIEWS2020Characterization of Protein-Membrane Interfaces through a Synergistic Computational-Experimental Approach
The characterization of biological interfaces is widely recognized as one of the main challenges for modern biology. In particular, biological membranes are nowadays known to be an active environment that allows membrane proteins to perform their work and ...
EPFL2020