Publication
BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies
Publications associées (37)
WHO IS INTERESTED IN THE REDUCTIVE DECHLORINATION OF POLYCHLORINATED BIPHENYLS (PCBs)?
Christof Holliger, Pierre Rossi, Martina Praveckova
PCBs are persistent organic pollutants which have entered the environment through both use and disposal. The environmental dissemination of PCBs is complex and nearly global in scale, although their production was banned in the 80’s. Nowadays, specific bac ...
2010Microfluidic assays for DNA manipulation based on a block copolymer immobilization strategy
Demetri Psaltis, Andreas Vasdekis
Methods to manipulate and visualize isolated DNA and oligonucleotide strands are important for investigation of their biophysics as well as their interactions with proteins. Herein, we report such a method by combining a block copolymer surface functionali ...
2010Interdigitated electrode DNA biosensor for detection of food-borne pathogens by impedance spectroscopy
The commercial application of biosensors has had a significant impact in a number of areas, particularly in the field of medical diagnostics. But in spite of the great number of publications, only a few systems are commercially available in food analysis. ...
EPFL2009Next-generation DNA sequencing techniques
Next-generation high-throughput DNA sequencing techniques are opening fascinating opportunities in the life sciences. Novel fields and applications in biology and medicine are becoming a reality, beyond the genomic sequencing which was original development ...
2009Methods of nucleic acid amplification and sequencing
Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified o ...
2008Organization of lin genes and IS6100 among different strains of hexachlorocyclohexane-degrading Sphingomonas paucimobilis: Evidence for horizontal gene transfer
Christof Holliger, Vishakha Raina
The organization of lin genes and IS6100 was studied in three strains of Sphingomonas paucimobilis (1390A, Sp+, and UT26) which degraded hexachlorocyclohexane (HCH) isomers but which had been isolated at different geographical locations. DNA-DNA hybridizat ...
2004A microfluidic platform using molecular beacon-based temperature calibration for thermal dehybridization of surface-bound DNA
Nico de Rooij, Gerardo Turcatti
This work presents a simple microfluidic device with an integrated thin-film heater for studies of DNA hybridization kinetics and double-stranded DNA melting temperature measurements. The heating characteristics of the device were evaluated with a novel, n ...
2004Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities
Christof Holliger, Julien Maillard, Katia Szynalski
We developed standard curves based on plasmids containing a 16S rRNA gene of a member of one of the three genera Dehalobacter, Desulfitobacterium, and Dehalococcoides. A large difference in amplification efficiency between the standard curves was observed ...
2004Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells
Florian Maria Wurm, Martin Jordan, Jason Wright
One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid D ...
2003Synthesis of selectively 15N-labeled 2'-O-{[(triisopropylsilyl)oxy]methyl}(=tom)-protected ribonucleoside phosphoramidites and their incorporation into a bistable 32mer RNA sequence
We present optimized reaction conditions for the conversion of 2'-O-{[(triisopropylsilyl)oxy]methyl}(tom) protected uridine and adenosine nucleosides into the corresponding protected (3-15N)-labeled uridine and cytidine and (1-15N)-labeled adenosine and gu ...
2003