Multilayer-Assembled Microchip for Enzyme Immobilization as Reactor Toward Low-Level Protein Identification
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Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent t ...
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Mass spectrometry (MS) has emerged over the last two decades as the analytical technique of choice in systems-level protein studies, known as proteomics. Two are the MS-based approaches generally applied to proteomics: bottom-up (BU), which relies on the p ...
A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...
A cleanroom-free fabrication process adapted to microfluidic devices is presented for pyrotechnical microelectromechanical systems balloon actuators. The actuators are intended for on-chip pumping and fluid ejection to replace tabletop pumping solutions in ...
Institute of Electrical and Electronics Engineers2014
The research areas of tissue engineering and drug development have displayed increased interest in organ-on-a-chip studies, in which physiologically or pathologically relevant tissues can be engineered to test pharmaceutical candidates. Microfluidic techno ...
We demonstrate a microfluidic method for ultra-sensitive protein detection in serum. First, ‘large’ (2.8 μm) antibody-functionalized magnetic beads specifically capture antigen from a serum matrix under active microfluidic mixing. Subsequently, the large b ...
Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...
Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...