Study of the improved Sf9 transient gene expression process

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Publications associées (31)

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We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

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Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

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Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

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Transient vitamin B5 starving improves mammalian cell homeostasis and protein production

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Maintaining a metabolic steady state is essential for an organism's fitness and survival when confronted with environmental stress, and metabolic imbalance can be reversed by exposing the organism to fasting. Here, we attempted to apply this physiological ...

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Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

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Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...

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Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription

Human T-cell lymphotropic virus type 1 (HTLV-1) Tax oncoprotein is required for viral gene expression. Tax transactivates the viral promoter by recruiting specific transcription factors but also by interfering with general transcription factors involved in ...

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Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

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Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent vectors. ...

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The role of optic flow pooling in insect flight control in cluttered environments

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Flight through cluttered environments, such as forests, poses great challenges for animals and machines alike because even small changes in flight path may lead to collisions with nearby obstacles. When flying along narrow corridors, insects use the magnit ...

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Flying robots are increasingly used for tasks such as aerial mapping, fast exploration, video footage and monitoring of buildings. Autonomous flight at low altitude in cluttered and unknown environments is an active research topic because it poses challen ...

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Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster

Bruno Lemaitre, Jan Paul Dudzic, Elodie Julie Virginie Ramond, Andrew Jonathan Bretscher, Claudia Melcarne

Eater and NimC1 are transmembrane receptors of the Drosophila Nimrod family, specifically expressed in haemocytes, the insect blood cells. Previous ex vivo and in vivoRNAi studies have pointed to their role in the phagocytosis of bacteria. Here, we have cr ...

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Analysis of the S. pombe Meiotic Proteome Reveals a Switch from Anabolic to Catabolic Processes and Extensive Post-transcriptional Regulation

Viesturs Simanis, Andrea Krapp, Romain Hamelin, Diego Chiappe, Florence Armand, Lucien Fabrice Krapp, Marc Moniatte, Elena Cano

Meiotic progression in S. pombe is regulated by stage-specific gene expression and translation, changes in RNA stability, expression of anti-sense transcripts, and targeted proteolysis of regulatory proteins. We have used SILAC labeling to examine the rela ...

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Analysis of the S. pombe Meiotic Proteome Reveals a Switch from Anabolic to Catabolic Processes and Extensive Post-transcriptional Regulation

Viesturs Simanis, Andrea Krapp, Romain Hamelin, Diego Chiappe, Florence Armand, Lucien Fabrice Krapp, Marc Moniatte, Elena Cano

2019