Unité
Laboratoire de microscopie électronique biologique
LaboratoirePublications associées (53)
Differential Visual Proteomics: Enabling the Proteome-Wide Comparison of Protein Structures of Single-Cells
Henning Paul-Julius Stahlberg, Anastasia Syntychaki
Proteins are involved in all tasks of life, and their characterization is essential to understand the underlying mechanisms of biological processes. We present a method called "differential visual proteomics" geared to study proteome-wide structural change ...
American Chemical Society (ACS)2019Structure and transformation of bacteriophage A511 baseplate and tail upon infection of Listeria cells
Sergey Nazarov, Petr Leiman, Ricardo Cesar Guerrero Ferreira
Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteri ...
WILEY2019Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy
Henning Paul-Julius Stahlberg, Ricardo Cesar Guerrero Ferreira, Pratibha Kumari
Intracellular inclusions rich in alpha-synuclein are a hallmark of several neuropathological diseases including Parkinson’s disease (PD). Previously, we reported the structure of alpha-synuclein fibrils (residues 1–121), composed of two protofibrils that a ...
2019Structural and functional analysis of host attachment proteins from contractile injection systems
Bacteriophages of the myoviridae family, R-type pyocin, Photorhabus virulence cassette (PVC), Serratia antifeeding prophage (Afp) and type 6 secretion system (T6SS) form a class of contractile injection systems that share common structural and functional c ...
EPFL2018Cytosolic delivery and characterization of monobodies interfering with SH2 domain-phosphotyrosine interactions
Targeted cancer therapy is a very promising concept; however, small molecule inhibitors only exist for a limited number of oncoproteins and resistance development limits their clinical use. Different engineered non-antibody scaffolds are being explored to ...
EPFL2018Inner-membrane GspF of the bacterial type II secretion system is a dimeric adaptor mediating pseudopilus biogenesis
The type II secretion system (T2SS), a protein complex spanning the bacterial envelope, is pivotal to bacterial pathogenicity. Central to T2SS function is the extrusion of protein cargos from the periplasm into the extracellular environment mediated by a p ...
2018Cryo-EM structure of alpha-synuclein fibrils
Henning Paul-Julius Stahlberg, Ricardo Cesar Guerrero Ferreira
Parkinson's disease is a progressive neuropathological disorder that belongs to the class of synucleinopathies, in which the protein alpha-synuclein is found at abnormally high concentrations in affected neurons. Its hallmark are intracellular inclusions c ...
eLife Sciences Publications, Ltd2018Proteoliposomes - a system to study membrane proteins under buffer gradients by cryo-EM
Membrane proteins are vital to life and major therapeutic targets. Yet, understanding how they function is limited by a lack of structural information. In biological cells, membrane proteins reside in lipidic membranes and typically experience different bu ...
Walter de Gruyter GmbH2017Studying membrane-protein interplay by modeling realistic conditions
Realistic models of cellular environments have long captured the imagination of biologists and physical scientists. More than 20 years ago, Goodshellâs inspiring rendering of cellular environments provided the foundation for the idea of capturing the ful ...
EPFL2017Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end
Henning Paul-Julius Stahlberg, Sergey Nazarov
The bacterial Type VI secretion system (T6SS) assembles from three major parts: a membrane complex that spans inner and outer membranes, a baseplate, and a sheath-tube polymer. The baseplate assembles around a tip complex with associated effectors and conn ...
EMBO2017