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Laboratoire d'ingénierie des protéines
LaboratoirePublications associées (937)
Solid-phase peptide synthesis in 384-well plates
Christian Heinis, Edward Jeffrey Will, Anne Sofie Luise Zarda, Alexander Lund Nielsen, Sevan Mleh Habeshian, Mischa Schüttel, Gontran Sangouard
Newer solid-phase peptide synthesis and release strategies enable the production of short peptides with high purity, allowing direct screening for desired bioactivity without prior chromatographic purification. However, the maximum number of peptides that ...
Hoboken2024Dietary L-Glu sensing by enteroendocrine cells adjusts food intake via modulating gut PYY/NPF secretion
Amino acid availability is monitored by animals to adapt to their nutritional environment. Beyond gustatory receptors and systemic amino acid sensors, enteroendocrine cells (EECs) are believed to directly percept dietary amino acids and secrete regulatory ...
Nature Portfolio2024Interrupted Polonovski Strategy for the Synthesis of Functionalized Amino Acids and Peptides
Jérôme Waser, Christine Alice Jacqueline Marty, Emmanuelle Madeline Dominique Allouche
The alpha-functionalization of carbamate-protected hydroxylamine glycine derivatives, acting as imine surrogates via an interrupted Polonovski reaction, is described to access functionalized amino acid derivatives. The addition of C, N, O, and S nucleophil ...
Washington2024De novo designed proteins: a study in engineering novel folds and functions
Alexandra Krina Van Hall-Beauvais
Proteins control nearly every facet of life on a molecular level. Proteins are formed from linear strings of amino acids, which fold into three-dimensional structures that can enact functions. Evolution has created highly efficient proteins in diverse fold ...
EPFL2023Synthesis and application of bifunctional hypervalent iodine reagents for peptide modification.
Protein-protein interactions (PPIs) are responsible for regulating many biological processes in human bodies. PPIs often have a large binding site and often interact through an alpha helical segment. Therefore, the inhibition of PPIs can be difficult using ...
EPFL2023Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins
Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge. Here we developed an original approa ...
NATURE PORTFOLIO2023De novo protein design by inversion of the AlphaFold structure prediction network
Bruno Emanuel Ferreira De Sousa Correia, Hamed Khakzad, Casper Alexander Goverde, Stéphane Rosset, Benedict Dieter Gregor Wolf
De novo protein design enhances our understanding of the principles that govern protein folding and interactions, and has the potential to revolutionize biotechnology through the engineering of novel protein functionalities. Despite recent progress in comp ...
2023Development of methods for the synthesis of large combinatorial libraries of macrocyclic compounds
Macrocycles have raised much interest in the pharmaceutical industry due to their ability to bind challenging targets while often still being able to cross membranes to reach intracellular proteins. However, the development of macrocyclic ligands to new di ...
EPFL2023Computational design of dynamic receptor—peptide signaling complexes applied to chemotaxis
Patrick Daniel Barth, Mahdi Hijazi, Aurélien Laurent Jean-Charles Oggier, Ana Paola Rico Villarreal, Robert Everett Jefferson, Andreas Füglistaler
Engineering protein biosensors that sensitively respond to specific biomolecules by triggering precise cellular responses is a major goal of diagnostics and synthetic cell biology. Previous biosensor designs have largely relied on binding structurally well ...
2023Computer-implemented design of peptide:receptor signaling complexes for enhanced chemotaxis
Patrick Daniel Barth, Robert Everett Jefferson
The present invention relates to a computer-implemented method for engineering the interaction between a protein and a cognate peptide that are capable of forming a molecular complex, wherein the method comprises (a) preparing in silico a library of test p ...
2023