DNA Replication and PCR Basics

This lecture covers the intricate process of DNA replication, detailing the roles of proteins like Helicase, DNA polymerase, and Primase in synthesizing new DNA strands. It also delves into DNA repair mechanisms, focusing on Nucleotide Excision Repair and the impact of UV-induced damage. Furthermore, the lecture explains the fundamentals of Polymerase Chain Reaction (PCR), illustrating how it amplifies targeted DNA sequences through multiple cycles. The genetic diversity between individuals is explored, highlighting Single Nucleotide Polymorphisms (SNPs) and microsatellites. Lastly, the lecture discusses the application of PCR in forensic science, emphasizing how DNA profiles can uniquely identify individuals.