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This lecture covers the process of nitrogen fixation, where organisms capture N₂ from the atmosphere to incorporate nitrogen into biomass. It explains the energy-intensive activation of N₂, the role of nitrogenase enzyme, and the mechanisms involved in transferring electrons to form NH3. The lecture also discusses the challenges of aerobic nitrogen fixation, the strategies used by cyanobacteria to deal with O₂ toxicity, and the importance of nitrogen fixation in maintaining soil nitrogen levels.