Lecture

Fluorescence: Principles and Applications

Description

This lecture covers the principles of fluorescence, including the Jablonski diagram, emission phenomena, and examples of fluorophores. It also discusses applications such as biomolecular analysis, NADH quantification, and FRET for measuring distances between fluorophores. Additionally, it explores the use of GFP in genetic engineering, FISH for DNA sequence detection, and energy transfer in hybridization detection.

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