Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.
Covers the introduction to fluorescence in microscopy, labeling techniques, specificity of markers, multi-color imaging, and factors affecting fluorescence properties.
Explores fluorescence lifetime in photomedicine, covering health effects of artificial light, fluorescence guided surgery, and applications of optical spectroscopy.