Lecture
DNA: PCR and Sequencing Techniques
Description
This lecture covers the basics of engineering with DNA, focusing on the polymerase chain reaction (PCR) method to amplify specific DNA samples. It explains the steps of denaturing, annealing, and synthesizing new DNA strands. The Sanger sequencing technique is also discussed, detailing the process of determining the nucleic acid sequence in DNA. Furthermore, the lecture delves into the Human Genome Project, highlighting key findings and the significance of mapping the human genome. It concludes with an overview of recombinant DNA technology, bacterial DNA manipulation, and the revolutionary CRISPR/Cas9 gene editing system.
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