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This lecture focuses on characterizing and classifying cells into different types based on various modalities, such as molecular profiles, morphology, electrophysiology, and functional response properties. The instructor discusses the power of single-cell RNA sequencing and transcriptomics in identifying cell types and differences in function, emphasizing the importance of validating distinctions. The field is rapidly evolving, with new papers regularly emerging to classify cell types in different brain regions, making this data modality essential for neuroscience research.