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This lecture explores the flow, transport, and mixing processes in confined media, focusing on pore scale velocity distribution, fluid flows through throat structures, particle tracking simulations, and diffusiophoresis effects. The instructor discusses the impact of pore throat size variability on low velocity distribution, the structure characterization of transmitting and dead-end pores, and the modeling of flow and transport through dead-end pores. Various experiments and simulations are presented to understand the behavior of colloids and bacteria in confined structures, shedding light on diffusion, biomass accumulation, and bacterial growth in porous environments.