Microscopy Objectives: Correction and Aberrations

This lecture covers the correction of major optical aberrations in microscopy, including spherical and chromatic aberration, coma, astigmatism, curvature of field, and distortion. It explains the design and performance of commonly used objectives like achromats, plan achromats, semi-apochromats (fluorites), and apochromats. The presentation delves into the correction of field curvature in objectives, the use of fluorite and semi-apochromat objectives, and the characteristics of apochromat objectives. It also discusses the importance of aberration correction in microscopy and the markings found on objective barrels, as well as the care and cleaning of optics to maintain image quality and prevent damage.