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This lecture covers the principles and applications of dielectrophoresis (DEP), which is the motion of polarizable particles in a non-uniform electric field. Topics include the polarization factor of particles, positive and negative DEP, dielectric models of cells, DEP sorting in flow, trapping devices, and the polarization factor of live and dead cells. The lecture also discusses the use of DEP for sorting and trapping cells, as well as differentiating between live and dead cells based on their response to DEP at various frequencies.