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This lecture explores the concept of symbiosis, focusing on commensalism, mutualism, and parasitism in microbial associations. It delves into the importance of symbiotic relationships in global health and agriculture, highlighting examples like the squid-vibrio light organ symbiosis. The lecture also discusses the evolution of eukaryotes through endosymbiosis, emphasizing the role of symbiotic microorganisms in nutrient provision and waste recycling. Furthermore, it covers the impact of facultative and obligate endosymbionts in insects, showcasing examples like Wolbachia and Spiroplasma. The lecture concludes by examining the potential applications of endosymbionts in disease control and conservation efforts.