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This lecture covers the three domains of life: Eukaryotes, Prokaryotes, and Archaea, focusing on their phylogenetic relationships and metabolic processes. It discusses the modes of nutrition, oxygen metabolism, and nitrogen metabolism in different organisms. The lecture also explores the concept of metabolic cooperation among prokaryotic cells and the mechanisms of genetic diversity acquisition through competence, conjugation, and transduction. Additionally, it delves into the human microbiome, highlighting the mutualistic relationship between humans and symbiotic microbes, and the impact of gut microbiota on human health.