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This lecture covers the important phenomenon of osmosis, its relevance in biology, and the concept of affinity illustrated through a practical experiment. The instructor explains the equilibrium state of an osmotic cell immersed in water, the role of an osmotic membrane, and the determination of osmotic pressure. The lecture delves into the analysis of chemical potentials, the application of Maxwell's relations, and the characterization of the variation of chemical potential with pressure. By exploring the pressure osmotic concept, the instructor provides a different perspective on osmosis, relating it to the behavior of a perfect gas under similar conditions.