Optical Filters: Understanding Properties and Selection

In course

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Description

This lecture covers the importance of understanding the properties of optical filters for fluorescence microscopy, including the characterization of transmission/reflection spectra and selecting suitable filter combinations. Examples are provided on choosing filter sets based on absorption and emission spectra, as well as the arrangement of filters in a fluorescence filter cube. The lecture also discusses multi-color imaging using different dyes and the use of filter sets to examine each dye separately.

Instructor

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Official source

https://mediaspace.epfl.ch/media/0_rd822kcw

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Ontological neighbourhood

Chemistry

Physical chemistry: Spectroscopy, Fluorescence imaging

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