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Lecture
Are you an EPFL student looking for a semester project?
Work with us on data science and visualisation projects, and deploy your project as an app on top of Graph Search.
This lecture covers the importance of understanding the properties of optical filters for fluorescence microscopy, including the characterization of transmission/reflection spectra and selecting suitable filter combinations. Examples are provided on choosing filter sets based on absorption and emission spectra, as well as the arrangement of filters in a fluorescence filter cube. The lecture also discusses multi-color imaging using different dyes and the use of filter sets to examine each dye separately.