Protein Engineering & In vitro Evolution

This lecture covers the fundamentals of protein engineering, including motivations, methodologies, and the challenges posed by the vast sequence space. It explores the use of phage display, ribosomal display, and yeast display for screening protein libraries. The lecture delves into the properties of non-immunoglobulin protein scaffolds and provides examples of their applications. It also discusses the computational design of proteins and the continuous directed evolution of biomolecules. The importance of screening conditions and library size in selecting proteins with desired properties is emphasized.