Protein Engineering & In Vitro Evolution

This lecture covers protein engineering, focusing on methodologies to engineer proteins, computational protein design, and screening protein libraries. It explores the motivations behind protein engineering, challenges due to the vast sequence space, and strategies like mutagenesis and library screening. The lecture also delves into in vitro affinity-selection of antibodies, generation of antibody phage libraries, and affinity-selection processes. Examples of protein scaffolds like Fibronectin and Ankyrin repeat protein are discussed, along with computational design and sequence library screening. The importance of screening conditions, structural complexity, and the use of in vitro evolution for reducing library size are highlighted.