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HPLC: Stationary Phases
This lecture covers the importance of solvent purity in HPLC, the role of eluents in chromatography, different types of stationary phases like silica and alumina, and the modification of silica for improved performance. It also discusses the use of reverse phases, the impact of eluents on solute retention, and the characteristics of various stationary phases. The lecture delves into the modification of stationary phases for better stability and selectivity, the mechanisms of solute retention, and the chromatographic separation of different compounds based on the nature of the stationary phase and eluent.