Size Exclusion Chromatography

This lecture covers the principles and applications of Size Exclusion Chromatography (SEC), also known as Gel Permeation Chromatography (GPC). SEC is used for analyzing macromolecules based on their molar masses, with the eluent typically being a single solvent. The lecture explains the interpretation of chromatographic data, the different types of columns and detectors used, as well as the stationary phases involved. It delves into the retention principles, distribution coefficients, and the factors influencing the separation process. Various detectors like refractive index, UV-visible, and light scattering are discussed, along with the importance of column temperature and sample injection volume. The lecture also touches upon the different types of gels used in SEC, the elution process, and the factors affecting resolution and selectivity.