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This lecture covers the concept of diffraction by a grating, which consists of parallel linear grooves that diffract light in specific directions. The diffraction spots on a screen indicate unique diffraction angles, leading to constructive interference. The dispersion effect of a grating is explained, showing how different orders of diffraction are related to wavelength. Additionally, the lecture discusses the use of diffraction gratings to disperse white light and analyze samples using UV-Vis spectroscopy. The relationship between diffraction angle, period size, and spatial frequency is explored, emphasizing how smaller features diffract more light. Examples of diffraction phenomena in microscopy are provided, illustrating how particle size influences light scattering and angle of spreading.