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Chromatographic Models: Theory and Applications
This lecture covers chromatographic models, including the concept of linear dispersion, chromatographic efficiency, and the van Deemter equation. It explains the factors affecting chromatographic signal broadening, such as diffusion, kinetics, and mass transfer. The lecture also delves into the importance of terms in the van Deemter equation, the influence of flow velocity on separation efficiency, and the transformation of chromatographic progress. Additionally, it discusses the thermodynamics of chromatography, detailing the interaction between solutes and stationary/mobile phases, the distribution constant, and the influence of temperature on separation processes.