Proteome Mapping & Electrophile Responsivity

This lecture delves into mapping the local proteome in living systems for drug target identification, focusing on spatial and functional omics technologies. It explores the concept of precision electrophile delivery and the role of lipid-derived electrophiles in precision medicine. The instructor discusses the challenges of mapping spatiotemporal reactivity and introduces cutting-edge tools like APEX/APEX2 for proximity mapping. The lecture also covers the concept of cysteome's spatiotemporal responsivity to nature's reactive signals, highlighting the importance of posttranslational modifications and functional biological signal output. Furthermore, it explores the use of electrophile-responsive protein degradation systems for on-target validation and signaling interrogation.