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This lecture delves into the intricate process of DNA replication, explaining the bidirectional nature of replication forks, the role of leading and lagging strands, and the significance of DNA polymerases. The lecture also covers the universal genetic code, the degeneracy of codons, and the importance of non-coding RNAs. Furthermore, it explores the PCR technique, its applications in DNA amplification, and its role in various fields such as forensics and genetic testing. The instructor demonstrates how PCR can detect specific DNA sequences by exploiting the complementary base pairing principle. The lecture concludes by discussing the practical implications of understanding DNA replication mechanisms and the potential of PCR technology in diverse scientific disciplines.