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Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Etienne Shaffer, Corinne Moratal
2010
Journal paper
Abstract

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy. (C) 2010 Optical Society of America

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