Concept

Immunohistochemistry

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The Consortium for Top-Down Proteomics (www.topdownproteomics.org) launched the present study to assess the current state of top-down mass spectrometry (TD MS) and middle-down mass spectrometry (MD MS) for characterizing monoclonal antibody (mAb) primary s ...

2020

Microscale physico-chemical interactions between hydrodynamically confined liquids and immersed biological surfaces

David Philipp Taylor

In contrast to conventional microfluidics, where liquids are typically manipulated within closed channels, open-space microfluidics has emerged as a new class of techniques that are well suited for ver-satile interaction with biological substrates. In part ...

EPFL2019

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

Martinus Gijs, Diego Gabriel Dupouy, Saska Brajkovic, Fabio Aimi

Breast cancer is a highly heterogeneous disease. The efficacy of tailored therapeutic strategies relies on the precise detection of diagnostic biomarkers by immunohistochemistry (IHC). Therefore, considering the increasing incidence of breast cancer cases, ...

2019

Virus lasers for biological detection

Gabriel Aeppli

The selective amplification of DNA in the polymerase chain reaction is used to exponentially increase the signal in molecular diagnostics for nucleic acids, but there are no analogous techniques for signal enhancement in clinical tests for proteins or cell ...

Springer2019

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Diego Gabriel Dupouy, Benjamin Pelz, Saska Brajkovic

BackgroundAnaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing f ...

BMC2018

Cell-based quantification of biomarkers from an ultra-fast microfluidic immunofluorescent staining: application to breast cancer cell lines

Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen

Immunohistochemistry (IHC) is one of the main techniques currently used in the clinics for biomarker characterization. It consists in colorimetric labeling with specific antibodies followed by microscopy analysis. The results are then used for diagnosis an ...

SPIE-INT SOC OPTICAL ENGINEERING2018

Improvement of breast cancer diagnosis through microfluidics

Huu Tuan Nguyen

In breast cancer, 15-20% of cases are reported with overexpression of human epidermal growth factor receptor 2 (HER2) that causes rapid cancer progression and poor prognosis. Fortunately, HER2-targeted therapy using specific antibodies such as Trastuzumab ...

EPFL2018

Computational Immunohistochemistry: Recipes for Standardization of Immunostaining

Nuri Murat Arar, Maria Gabrani, Aditya Kashyap, Pushpak Pati, Anna Fomitcheva Khartchenko

Cancer diagnosis and personalized cancer treatment are heavily based on the visual assessment of immunohistochemically-stained tissue specimens. The precision of this assessment depends critically on the quality of immunostaining, which is governed by a nu ...

2017

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Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demons ...

Nature Publishing Group2017

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Fluorescence in situ hybridization (FISH) is one of the recommended techniques for human epidermal growth factor receptor 2 (HER2) status assessment on cancer tissues. Here we develop microfluidics-assisted FISH (MA-FISH), in which hybridization of the FIS ...

Nature Publishing Group2017