Concept
Immunohistochemistry
Related publications (66)
Fluorogenic polymethine dyes by intramolecular cyclization
Pablo Rivera Fuentes, Annabell Martin
Fluorescence imaging plays a pivotal role in the study of biological processes, and cell -permeable fluorogenic dyes are crucial to visualize intracellular structures with high specificity. Polymethine dyes are vitally important fluorophores in singlemolec ...
Elsevier Sci Ltd2024Solid-phase peptide synthesis in 384-well plates
Christian Heinis, Edward Jeffrey Will, Anne Sofie Luise Zarda, Alexander Lund Nielsen, Sevan Mleh Habeshian, Mischa Schüttel, Gontran Sangouard
Newer solid-phase peptide synthesis and release strategies enable the production of short peptides with high purity, allowing direct screening for desired bioactivity without prior chromatographic purification. However, the maximum number of peptides that ...
Hoboken2024Development of next-generation microfluidic systems for enhanced, faster, and cost-effective immunoassays for tissue diagnostics - Ac electrothermal flow & Acoustofluidics
The application of microfluidics in the field of surface-based assays and more specifically, the spatial molecular profiling of tumor tissues has gained a lot of interest, especially with the increased interest in personalized medicine and targeted therapy ...
EPFL2023Correlative light and volume electron microscopy to study brain development
Recent advances in volume electron microscopy (EM) have been driving our thorough understanding of the brain architecture. Volume EM becomes increasingly powerful when cells and their subcellular structures that are imaged in light microscopy are correlate ...
OXFORD UNIV PRESS2023Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
The usage of microfluidics for automated and fast immunoassays has gained a lot of interest in the last decades. This integration comes with certain challenges, like the reconciliation of laminar flow patterns of micro-scale systems with diffusion-limited ...
ROYAL SOC CHEMISTRY2023A general strategy to develop fluorogenic polymethine dyes for bioimaging
Pablo Rivera Fuentes, Annabell Martin
Fluorescence imaging is an invaluable tool to study biological processes and further progress depends on the development of advanced fluorogenic probes that reach intracellular targets and label them with high specificity. Excellent fluorogenic rhodamine d ...
Berlin2023Efficient AC electrothermal flow (ACET) on-chip for enhanced immunoassays
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
Biochemical reaction rates in microfluidic systems are known to be limited by the diffusional transport of reagents, leading often to lowered sensitivity and/or longer detection times in immunoassays. Several methods, including electrically powering electr ...
ROYAL SOC CHEMISTRY2023Dopamine Transporter, PhosphoSerine129 alpha-Synuclein and alpha-Synuclein Levels in Aged LRRK2 G2019S Knock-In and Knock-Out Mice
Salvatore Novello, Chiara Domenicale
The G2019S mutation in leucine rich-repeat kinase 2 (LRRK2) is a major cause of familial Parkinson's disease. We previously reported that G2019S knock-in mice manifest dopamine transporter dysfunction and phosphoSerine129 alpha-synuclein (pSer129 alpha-syn ...
MDPI2022Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy
Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...
AMER CHEMICAL SOC2021Synchrotron-based phase-contrast micro-CT as a tool for understanding pulmonary vascular pathobiology and the 3-D microanatomy of alveolar capillary dysplasia
This study aimed to explore the value of synchrotron-based phase-contrast microcomputed tomography (micro-CT) in pulmonary vascular pathobiology. The microanatomy of the lung is complex with intricate branching patterns. Tissue sections are therefore diffi ...
AMER PHYSIOLOGICAL SOC2020