Pipette

Summary

A pipette (sometimes spelled as pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the instrument. History The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required. The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). The founder of the company Eppendorf, Dr. Heinrich Netheler, inherited the rights and started

Official source

https://en.wikipedia.org/wiki/Pipette

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Impedance-Based Single-Cell Pipetting

Yann Barrandon, Nicolas Jean Bernard Beuchat, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Philippe Renaud, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability.

SAGE PUBLICATIONS INC2020

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A connector for providing both a fluidic and electrical connection is disclosed, said connector having a proximal end, a distal end and an elongated body in between, characterized in that the elongated body has an inner cavity spanning throughout its length, and the distal end comprises: a) an inner body portion comprising a first inner electrical contact and b) an outer body portion comprising a second outer electrical contact having a spring element. The connector is easily adaptable to many kind of fluidic actuators and particularly to pipette instruments usually found in laboratory practice and developed to adapt fluidic actuators and pipette instalments to work according to the Coulter principle in every working condition, in particular to adapt the electrical and fluidic connection between a sensing tip and an instrumented pipette.

2018

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The present invention relates to a pipette tip comprising a thin holed membrane at its distal end, which is designed to be adapted with a system comprising at least an impedance analyser and a fluidic actuator to perform the dispensing and analysis of particles comprised within a conductive medium by exploiting the Coulter counter principle. The pipette tip can comprise attached or floating electrodes at its internal or external side for creating an electrical circuit. Also disclosed therein is a dispensing and analysis system, methods of using thereof and pipette tip's manufacturing methods.

2016