Carcinoma in situ | EPFL Graph Search

Carcinoma in situ (CIS) is a group of abnormal cells. While they are a form of neoplasm, there is disagreement over whether CIS should be classified as cancer. This controversy also depends on the exact CIS in question (i.e. cervical, skin, breast). Some authors do not classify them as cancer, however, recognizing that they can potentially become cancer. Others classify certain types as a non-invasive form of cancer. The term "pre-cancer" has also been used. These abnormal cells grow in their normal place, thus "in situ" (from Latin for "in its place"). For example, carcinoma in situ of the skin, also called Bowen's disease, is the accumulation of dysplastic epidermal cells within the epidermis only, that has failed to penetrate into the deeper dermis. For this reason, CIS will usually not form a tumor. Rather, the lesion is flat (in the skin, cervix, etc.) or follows the existing architecture of the organ (in the breast, lung, etc.). Exceptions include CIS of the

GLUT3 is induced during epithelial-mesenchymal transition and promotes tumor cell proliferation in non-small cell lung cancer

Bart Deplancke, Svenja Johanna Groeneveld, Mark Jonathan Masin, Etienne Meylan, Natasha Ella Samson, Petra Catalina Schwalie, Jessica Christine Vazquez

Alterations in glucose metabolism and epithelial-mesenchymal transition (EMT) constitute two important characteristics of carcinoma progression toward invasive cancer. Despite an extensive characterization of each of them separately, the links between EMT and glucose metabolism of tumor cells remain elusive. Here we show that the neuronal glucose transporter GLUT3 contributes to glucose uptake and proliferation of lung tumor cells that have undergone an EMT.

2014

Time-Resolved Autofluorescence Spectroscopy of the Bronchial Mucosa for the Detection of Early Cancer: Ex vivo and Clinical Results

Tanja Gabrecht, Pascal Uehlinger, Hubert van den Bergh, Georges Wagnières

Time-resolved measurements of endogenous tissue autofluorescence were carried out on the bronchial mucosa of 18 patients during endoscopy by the means of a optical fibre-based spectrometer. The objective was to assess the fluorescence lifetime as a new contrast parameter between normal and malignant tissue and to explain the origin of a previously observed contrast in fluorescence intensity. The intra- and interpatient variation of tissue autofluorescence intensity and decay on normal tissue was determined with the outcome that a strong fluctuation in autofluorescence intensity but not in lifetime was observed on the normal tissue. Preliminary results were obtained by comparing fluorescence decays on normal mucosa and dysplasia/carcinoma in situ. No significant change in fluorescence decay nor in spectrum between 510 and 650 nm was found. Measurements in parallel with an endoscopic autofluorescence imaging device, on the other hand, indicated a contrast in intensity and spectrum on the same lesions. This suggests that the spectral contrast might be due to an enhanced blood concentration in deeper lying layers of the lesion the optical fibre-based contact measurements are less sensitive to. The difference in intensity might be due to a lower concentration in fluorophores or to the thickening of the epithelium in the neoplastic mucous membrane. However, no indication for fluorescence quenching in the upper layers of the mucous membrane as the reason for the reduced fluorescence intensity was found. The fluorescence decays showed a quite stable behaviour with three decay times of 6.9 ns, 2.0 ns and 0.2 ns in the spectral range between 430 and 680 nm. This can be an indication that there is one dominant fluorophore involved, the calculated decay times suggest that it might be elastin. However, a slight spectral dependence of the fluorescence decays let presume that there is a contribution from other fluorophores, probably flavins and NADH.

2001

Time-resolved autofluorescence spectroscopy of the bronchial mucosa for the detection of early cancer: Clinical results

Tanja Gabrecht, Pascal Uehlinger, Hubert van den Bergh, Georges Wagnières

Time-resolved measurements of endogenous tissue autofluorescence were carried out on the bronchial mucosa of 18 patients during endoscopy by the means of an optical fibre-based spectrometer. The objective was to assess the fluorescence lifetime as a new contrast parameter between normal and malignant tissue and to explain the origin of a previously observed contrast in fluorescence intensity. The intra- and interpatient variation of tissue autofluorescence intensity and decay on normal tissue was determined, with the outcome that a strong fluctuation in autofluorescence intensity but not in lifetime was observed on the normal tissue. Preliminary results were obtained by comparing fluorescence decays on normal mucosa and dysplasia/carcinoma in situ. No significant change in fluorescence decay nor in spectrum between 510 and 650 nm was found. Measurements in parallel with an endoscopic autofluorescence imaging device, on the other hand, indicated a contrast in intensity and spectrum on the same lesions. This suggests that the spectral contrast might be due to an enhanced blood concentration in deeper-lying layers of the lesion to which the optical fibre-based contact measurements are less sensitive. The difference in intensity might be due to a lower concentration in fluorophores or to the thickening of the epithelium in the neoplastic mucous membrane. However, no indication of fluorescence quenching in the upper layers of the mucous membrane was found as the reason for the reduced fluorescence intensity. The fluorescence decays showed a quite stable behaviour with three decay times of 6.9 ns, 2.0 ns and 0.2 ns in the spectral range between 430 and 680 nm. This can be an indication that there is one dominant fluorophore involved, the calculated decay times suggest that it might be elastin. However, a slight spectral dependence of the fluorescence decays lead to the presumption that there is a contribution from other fluorophores, probably flavins and NADH.

2001

Time-resolved autofluorescence spectroscopy of the bronchial mucosa for the detection of early cancer: Clinical results

Tanja Gabrecht, Pascal Uehlinger, Hubert van den Bergh, Georges Wagnières

2001

Time-Resolved Autofluorescence Spectroscopy of the Bronchial Mucosa for the Detection of Early Cancer: Ex vivo and Clinical Results

Tanja Gabrecht, Pascal Uehlinger, Hubert van den Bergh, Georges Wagnières

2001