Concept
Optical microscope
Related concepts (37)
Magnifying glass
A magnifying glass is a convex lens that is used to produce a magnified of an object. The lens is usually mounted in a frame with a handle. A magnifying glass can be used to focus light, such as to concentrate the sun's radiation to create a hot spot at the focus for fire starting. A sheet magnifier consists of many very narrow concentric ring-shaped lenses, such that the combination acts as a single lens but is much thinner. This arrangement is known as a Fresnel lens.
Bright-field microscopy
Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique.
Phase-contrast microscopy
NOTOC Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium.
Eyepiece
An eyepiece, or ocular lens, is a type of lens that is attached to a variety of optical devices such as telescopes and microscopes. It is named because it is usually the lens that is closest to the eye when someone looks through an optical device to observe an object or sample. The objective lens or mirror collects light from an object or sample and brings it to focus creating an image of the object. The eyepiece is placed near the focal point of the objective to magnify this image to the eyes.
Virtual image
In optics, an image is defined as the collection of focus points of light rays coming from an object. A is the collection of focus points made by converging rays, while a virtual image is the collection of focus points made by extensions of diverging rays. In other words, a virtual image is found by tracing real rays that emerge from an optical device (lens, mirror, or some combination) backward to perceived or apparent origins of ray divergences.
Electron microscope
An electron microscope is a microscope that uses a beam of electrons as a source of illumination. They use electron optics that are analogous to the glass lenses of an optical light microscope. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light, electron microscopes have a higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes.
Near-field scanning optical microscope
Near-field scanning optical microscopy (NSOM) or scanning near-field optical microscopy (SNOM) is a microscopy technique for nanostructure investigation that breaks the far field resolution limit by exploiting the properties of evanescent waves. In SNOM, the excitation laser light is focused through an aperture with a diameter smaller than the excitation wavelength, resulting in an evanescent field (or near-field) on the far side of the aperture.
Diaphragm (optics)
In optics, a diaphragm is a thin opaque structure with an opening (aperture) at its center. The role of the diaphragm is to stop the passage of light, except for the light passing through the aperture. Thus it is also called a stop (an aperture stop, if it limits the brightness of light reaching the focal plane, or a field stop or flare stop for other uses of diaphragms in lenses). The diaphragm is placed in the light path of a lens or objective, and the size of the aperture regulates the amount of light that passes through the lens.
Accademia dei Lincei
The Accademia dei Lincei (akkaˈdɛːmja dei linˈtʃɛi; literally the "Academy of the Lynx-Eyed", but anglicised as the Lincean Academy) is one of the oldest and most prestigious European scientific institutions, located at the Palazzo Corsini on the Via della Lungara in Rome, Italy. Founded in the Papal States in 1603 by Federico Cesi, the academy was named after the lynx, an animal whose sharp vision symbolizes the observational prowess that science requires.
Immunofluorescence
Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. The specific region an antibody recognizes on an antigen is called an epitope.