Concept

Gel electrophoresis

Related publications (234)

A new non-toxic chemically cross-linked ELP hydrogel for in situ protein delivery

Proteins play an essential role in all biological processes and have been attracting more and more attention as drug candidates due to their high specificity and activity. However, their use as therapeutics has been seriously held back given that their abi ...

2009

Viscoelastic and poroelastic mechanical characterization of hydrated gels

Matteo Galli

Measurement of the mechanical behavior of hydrated gels is challenging due to a relatively small elastic modulus and dominant time-dependence compared with traditional engineering materials. Here polyacrylamide gel materials are examined using different te ...

Materials Research Society2009

Influence of Structural Variation on the Anticancer Activity of RAPTA-Type Complexes: ptn versus pta

Paul Joseph Dyson, Christian Hartinger, Alexey Nazarov, Anna Katherine Renfrew, Andrew Douglas Phillips

[M(η6-arene)(ptn)Cl]X (M = Ru, Os; arene = p-cymene, benzene, toluene, hexamethylbenzene; ptn = 3,7-dimethyl-7-phospha-1,3,5-triazabicyclo[3.3.1]nonane; X = Cl-, BF4-) were prepd. and characterized spectroscopically. X-ray diffraction was addnl. used to ch ...

2009

A Novel Cytotoxic Cerium Complex: Aquatrichloridobis(1,10-phenanthroline)cerium(III) (KP776). Synthesis, Characterization, Behavior in H2O, Binding towards Biomolecules, and Antiproliferative Activity

Michael Groessl

The lanthanide complex aquatrichloridobis(1,10-phenanthroline)cerium(III) [Ce(phen)(2)(H2O)Cl-3] (KP776) was fully characterized by elemental analysis, IR-, and H-1- and C-13-NMR spectroscopy, as well as TG/DTA measurements, and its behavior in H2O, import ...

2009

Effect of calcification on agarose gel stiffness and integration strength with Bone

Jérôme Hollenstein

The zone of calcified cartilage (ZCC) is critical for the normal attachment of articular cartilage to bone as well as to the biomimetic bioengineering of osteochondral tissue constructs. However, relatively few osteochondral tissue engineering approaches h ...

2009

Selective cross-linking of interacting proteins using self-labeling tags

Kai Johnsson, Grazvydas Lukinavicius, Kui Thong Tan

We have designed molecules that permit the selective cross-linking (S-CROSS) of interacting proteins in cell lysates and the sensitive detection of the trapped complexes through in-gel fluorescence scanning. S-CROSS requires the expression of the putative ...

American Chemical Society2009

An agarose-based microfluidic platform with a gradient buffer for 3D chemotaxis studies

Melody Swartz, Ulrike Haessler, Mingming Wu

The current state-of-art in 3D microfluidic chemotaxis device (mu FCD) is limited by the inherent coupling of the fluid flow and chemical concentration gradients. Here, we present an agarose-based 3D mu FCD that decouples these two important parameters, in ...

Springer Verlag2009

Statistical assessment of the variability of the T-RFLP analysis applied to complex microbial communities

Christof Holliger, Pierre Rossi, Emmanuelle Rohrbach, François Gillet

The variability of the T-RFLP analysis applied to complex microbial communities was assessed statistically. Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a ...

American Society for Microbiology2009

Kinetics of Proteolytic Reactions in Nanoporous Materials

Hubert Girault, Liang Qiao, Hongyan Bi

Proteolysis with proteases preloaded within nanopores of porous material is a very fast process, where proteins can be digested in minutes compared to the conventional bulk enzyme reactions taking place over hours. To model this surprising phenomenon, a mo ...

American Chemical Society2009

Basic principles of electrolyte chemistry for microfluidic electrokinetics. Part II: Coupling between ion mobility, electrolysis, and acid-base equilibria

Alexandre Louis André Persat

We present elements of electrolyte dynamics and electrochemistry relevant to microfluidic electrokinetics experiments. In Part I of this two-paper series, we presented a review and introduction to the fundamentals of acid-base chemistry. Here, we first sum ...

Royal Society of Chemistry2009

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