Concept

Phage display

Related publications (90)

Phage Selection of Bicyclic Peptide Ligands and Development of a New Peptide Cyclisation Method

Bicyclic peptides binding to targets of interest can be isolated from combinatorial libraries using a phage display-based approach. In a first project of this thesis, we aimed at generating bicyclic peptide inhibitors of matrix metalloproteinases (MMPs), a ...

EPFL2012

Contractile Tail Machines of Bacteriophages

Petr Leiman

Bacteriophages with contractile tails epitomize the concepts of "virus" and "phage" for many because the tails of these phages undergo a large conformational change - resembling the action of a syringe - upon the attachment to the host cell. The contractil ...

2012

From Phage Display to Dendrimer Display: Insights into Multivalent Binding

Maartje Martina Cornelia Bastings

Phage display is widely used for the selection of target-specific peptide sequences. Presentation of phage peptides on a multivalent platform can be used to (partially) restore the binding affinity. Here, we present a detailed analysis of the effects of va ...

American Chemical Society2011

Post-translational modification of genetically encoded polypeptide libraries

Christian Heinis, Alessandro Angelini

The genetic encoding of polypeptides with biological display systems enables the facile generation and screening of very large combinatorial libraries of molecules. By post-translationally modifying the encoded polypeptides, chemically and structurally mor ...

Elsevier2011

Automated protein-DNA interaction screening of Drosophila regulatory elements

Bart Deplancke, Andreas Massouras, Alina Isakova, Julien Bryois, Jean-Daniel Feuz, Antonina Iagovitina

Drosophila melanogaster has one of the best characterized metazoan genomes in terms of functionally annotated regulatory elements. To explore how these elements contribute to gene regulation, we need convenient tools to identify the proteins that bind to t ...

2011

Nanomechanics of Streptavidin Hubs for Molecular Materials

Fabrizio Benedetti

A new strategy is reported for creating protein-based nanomaterials by genetically fusing large polypeptides to monomeric streptavidin and exploiting the propensity of streptavidin monomers(SM) to self-assemble into stable tetramers. We have characterized ...

2011

Microfluidic assays for DNA manipulation based on a block copolymer immobilization strategy

Demetri Psaltis, Andreas Vasdekis

Methods to manipulate and visualize isolated DNA and oligonucleotide strands are important for investigation of their biophysics as well as their interactions with proteins. Herein, we report such a method by combining a block copolymer surface functionali ...

2010

High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics

Christoph Merten

During the last 25 years, display techniques such as phage display have become very powerful tools for protein engineering, especially for the selection of monoclonal antibodies. However, while this method is extremely efficient for affinity-based selectio ...

2010

Retroviral Display in Gene Therapy, Protein Engineering, and Vaccine Development

Christoph Merten

The display and analysis of proteins expressed on biological surfaces has become an attractive tool for the study of molecular interactions in enzymology, protein engineering, and high-throughput screening. Among the growing number of established display s ...

2010

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

Oliver Hantschel

Interactions between Src homology 2 (SH2) domains and phosphotyrosine sites regulate tyrosine kinase signaling networks. Selective perturbation of these interactions is challenging due to the high homology among the 120 human SH2 domains. Using an improved ...

2010

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