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Concept
Azotobacter
Summary
Azotobacter is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil (nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, Azotobacter chroococcum, was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. Azotobacter species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants.
Cells of the genus Azotobacter are relatively large for bacteria (2–4 μm in diameter). They are usually oval, but may take various forms from rods to spheres. In microscopic preparations, the cells can be dispersed or form irregular clusters or occasionally chains of varying lengths. In fresh cultures, cells are mobile due to the numerous flagella. Later, the cells lose their mobility, become almost spherical, and produce a thick layer of mucus, forming the cell capsule. The shape of the cell is affected by the amino acid glycine, which is present in the nutrient medium peptone.
Under magnification, the cells show inclusions, some of which are colored. In the early 1900s, the colored inclusions were regarded as "reproductive grains", or gonidia – a kind of embryo cells. However, the granules were later determined to not participate in the cell division. The colored grains are composed of volutin, whereas the colorless inclusions are drops of fat, which act as energy reserves.
Cysts of the genus Azotobacter are more resistant to adverse environmental factors than the vegetative cells; in particular, they are twice as resistant to ultraviolet light.
Official source
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